14-9819-82

antibody from Invitrogen Antibodies
Targeting: SPI1 OF, PU.1, SFPI1, SPI-1, SPI-A
Provider product page for 14-9819-82
Western blot
Immunohistochemistry

Antibody data

Product number
14-9819-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
PU.1 Monoclonal Antibody (phpu13), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal phpu13 recognizes human and mouse PU.1, a member of the ets family of transcription factors. This 42 kDa protein was originally identified as Spi-1, a proto-oncogene, for its role in Friend virus-induced murine erythroleukemia. Expression of PU.1 plays a critical role in lymphocyte and myeloid development. Knock-out mice are neonatal lethal and lack myeloid, T and B cells. In addition erythroid development is also altered. Protein expression levels help guarantee proper development; higher levels in myeloid cells compared to lymphocytes. PU.1 controls transcription of several proteins including CD11b, CD18, Gata2, IRF4, CD20, CD10, and CD79. Disruption of PU.1 expression may play a role in several specific leukemias; such as B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, and nodular lymphocyte predominant Hodgkin. Applications Reported: This phpu13 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections and immunocytochemistry. Applications Tested: This phpu13 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58). This can be used at less than or equal to 10 µg/mL. It is recommeded that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Host
Mouse
Isotype
IgG
Antibody clone number
phpu13
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
SPI1 protein structure - 14-9819-82 shown in red.