LS-C782440

antibody from LSBio

Targeting: SPI1 OF, PU.1, SFPI1, SPI-1, SPI-A

Western blot (Enhanced validation)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C782440

Provider

LSBio

Product name

SPI1 / PU.1 Antibody (clone PU1/2146) LS-C782440

Antibody type

Monoclonal

Description

Protein G affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

PU1/2146

Storage

Store at 2-8°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot testing of human THP-1 cell lysate with PU.1 antibody (clone PU1/2146). Predicted molecular weight ~31 kDa but routinely observed at ~40 kDa.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC staining of FFPE human lymph node with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC staining of FFPE Hodgkins lymphoma with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC staining of FFPE human lymph node with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC staining of FFPE Hodgkins lymphoma with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC staining of FFPE human colon carcinoma with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC staining of FFPE human spleen with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC staining of FFPE human spleen with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC staining of FFPE human lymph node with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC staining of FFPE Hodgkins lymphoma with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC staining of FFPE human colon carcinoma with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.