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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 6688-MSM2-P0 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PU.1 (SPI-1) Monoclonal Antibody (PU1, 2146)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- PU1, 2146
- Vial size
- 20 µg
- Concentration
- 200 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of THP-1 cell lysate using PU.1 (SPI-1) Monoclonal Antibody (Product # 6688-MSM2-P1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of PFA-fixed Ramos cells using PU.1 (SPI-1) Monoclonal Antibody (Product # 6688-MSM2-P1) followed by goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human colon carcinoma using PU.1 (SPI-1) Monoclonal Antibody (Product # 6688-MSM2-P1).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human Hodgkins lymphoma using PU.1 (SPI-1) Monoclonal Antibody (Product # 6688-MSM2-P1).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human lymph node using PU.1 (SPI-1) Monoclonal Antibody (Product # 6688-MSM2-P1).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human spleen using PU.1 (SPI-1) Monoclonal Antibody (Product # 6688-MSM2-P1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of PFA-fixed Ramos cells using PU.1 (SPI-1) Monoclonal Antibody (Product # 6688-MSM2-P1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Analysis of Protein Array containing more than 19,000 full-length human proteins PU.1 (SPI-1) Monoclonal Antibody (Product # 6688-MSM2-P1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDS-PAGE Analyis Purified PU.1 (SPI-1) Monoclonal Antibody (Product # 6688-MSM2-P1). Confirmation of Purity and Integrity of Antibody.
