V8948

antibody from NSJ Bioreagents

Targeting: SPI1 OF, PU.1, SFPI1, SPI-1, SPI-A

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V8948

Provider

NSJ Bioreagents

Product name

Recombinant PU.1 Antibody / SPI1

Antibody type

Monoclonal

Description

This highly specific Recombinant PU.1 antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

rPU1/2146

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the recombinant PU.1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lymph node with recombinant PU.1 antibody (clone rPU1/2146). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lymph node tissue with recombinant PU.1 antibody (clone rPU1/2146). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free recombinant PU.1 antibody (clone rPU1/2146) as confirmation of integrity and purity.