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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP1-00135 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-00135, RRID:AB_1504200
- Product name
- Goat Polyclonal PU.1/Spi-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoforms (NP_001074016.1
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
internal region- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Upregulation of the neuron-specific K+/Cl- cotransporter expression by transcription factor early growth response 4.
The hematopoietic transcription factor PU.1 represses gelatinase A transcription in glomerular mesangial cells.
Uvarov P, Ludwig A, Markkanen M, Rivera C, Airaksinen MS
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Dec 27;26(52):13463-73
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Dec 27;26(52):13463-73
The hematopoietic transcription factor PU.1 represses gelatinase A transcription in glomerular mesangial cells.
Harendza S, Lovett DH, Stahl RA
The Journal of biological chemistry 2000 Jun 30;275(26):19552-9
The Journal of biological chemistry 2000 Jun 30;275(26):19552-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PU.1/Spi-1 Antibody [NBP1-00135] - (0.03ug/ml) Analysis of DAUDI lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP1-00135] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PU.1/Spi-1 Antibody [NBP1-00135] - Analysis of PU.1/Spi1 in p3xFLAG-PU.1 transfected 293T cells (1 million cells) using anti-PU.1/Spi1 antibody. Image from verified customer review.
