21166-1-AP

antibody from Proteintech Group

Targeting: MRTFA BSAC, KIAA1438, MAL, MKL, MKL1, MRTF-A

Provider product page for 21166-1-AP

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Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [44]
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Validations
Western blot [1]

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Product number: 21166-1-AP - Provider product page
Provider: Proteintech Group
Product name: MKL1 antibody
Antibody type: Polyclonal
Description: KD/KO validated MKL1 antibody (Cat. #21166-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MRTFA protein structure - 21166-1-AP shown in red.

Submitted references MRCKα Is a Suppressor of GEF-H1/RhoA/MRTF Signaling in Tubular Cells.

Sri Theivakadadcham VS, Dan Q, Wu B, Venugopal S, Maksimoska V, Yucel-Polat A, Kapus A, Szászi K
Cells 2026 Mar 2;15(5)

ARPC2 Promotes Pulmonary Fibrosis by Regulating MRTFA Activity Independent of the Canonical ARP2/3 Complex.

Du EJ, Kim H, Bae SG, An S, Ryoo K
International journal of molecular sciences 2026 Mar 17;27(6)

Regulatory role of MRTF-A in macrophage polarization and the therapeutic mechanism of Bushen Wenyang Huayu decoction in endometriosis.

Meng X, Qi J, Yang X, Zhang L, Fu X, Li J, Chen J
Journal of ethnopharmacology 2026 Jul 15;366:121602

VAP1 promotes cardiac fibrosis by enabling PDGFR signaling in myofibroblasts.

Huang S, Zhao Q, Shao T, Zhu C, Xue Y, Chen N, Zhang Y, Xu H, Kong M, Wang R
Experimental & molecular medicine 2026 Apr;58(4):1284-1296

Methyltransferase-like 3-catalysed N6-methyladenosine methylation facilitates the contribution of vascular smooth muscle cells to atherosclerosis.

Dong Z, Jin Y, Shen Y, Huang J, Tan J, Feng Q, Gong Z, Zhu S, Chen H, Yu F, Li W, Jia Y, Kong W, Fu Y
Cardiovascular research 2025 May 6;121(4):568-584

Serum starvation-induced cholesterol reduction increases melanoma cell susceptibility to cytotoxic T lymphocyte killing.

Hou M, Ji L, Li D, Xiao Q, Hu X
Scientific reports 2025 May 26;15(1):18364

Activation of megakaryocytic leukemia 1 in endothelial cells contributes to diabetic retinopathy in mice.

Zhu Y, Feng X, Wang F, Ding Y
Life sciences 2025 Mar 1;364:123425

Ubiquitin Specific Protease 9X Regulates the Activation of ARK5 and Promotes Progression of Fibrotic Remodeling.

Li X, Jiang S, Yang W, Zhu X, Zhang F, Li Z, Guo X, Wei Y
JACC. Basic to translational science 2025 Jul;10(7):101255

Mechanistic insights into the treatment of pulmonary fibrosis with bioactive components from traditional chinese medicine via matrix stiffness-mediated EMT.

Li K, Liu H, Li M, Sun M, Peng X, Wu Y, Tian Y, Liu X, Li J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Jan;136:156266

Atractylenolide-III restrains cardiac fibrosis after myocardial infarction via suppression of the RhoA/ROCK1 and ERK1/2 pathway.

Li X, Zhu X, Jiang S, Yang W, Zhang F, Guo X, Wei Y
International immunopharmacology 2025 Jan 3;145:113825

Photo-tunable hydrogels reveal cellular sensing of rapid rigidity changes through the accumulation of mechanical signaling molecules.

Yang J, Wang P, Zhang Y, Zhang M, Sun Q, Chen H, Dong L, Chu Z, Xue B, Hoff WD, Zhao C, Wang W, Wei Q, Cao Y
Cell stem cell 2025 Jan 2;32(1):121-136.e6

Matrix stiffness regulates nucleus pulposus cell glycolysis by MRTF-A-dependent mechanotransduction.

Xu H, Wei K, Ni J, Deng X, Wang Y, Xiang T, Song F, Wang Q, Niu Y, Jiang F, Wang J, Sheng L, Dai J
Bone research 2025 Feb 14;13(1):23

Anti-fibrogenic effect of umbilical cord-derived mesenchymal stem cell-conditioned media in human esophageal fibroblasts.

Choi YJ, Kim JH, Lee Y, Pyeon HJ, Yoo IK, Yoo JH
Scientific reports 2024 Sep 27;14(1):22233

Human umbilical cord/placenta mesenchymal stem cell conditioned medium attenuates intestinal fibrosis in vivo and in vitro.

Choi YJ, Kim WR, Kim DH, Kim JH, Yoo JH
Stem cell research & therapy 2024 Mar 7;15(1):69

Alarmin S100A8 imparts chemoresistance of esophageal cancer by reprogramming cancer-associated fibroblasts.

Chen X, Cheng G, Zhu L, Liu T, Yang X, Liu R, Ou Z, Zhang S, Tan W, Lin D, Wu C
Cell reports. Medicine 2024 Jun 18;5(6):101576

Regulation of the RhoA exchange factor GEF-H1 by profibrotic stimuli through a positive feedback loop involving RhoA, MRTF, and Sp1.

Venugopal S, Dan Q, Sri Theivakadadcham VS, Wu B, Kofler M, Layne MD, Connelly KA, Rzepka MF, Friedberg MK, Kapus A, Szászi K
American journal of physiology. Cell physiology 2024 Aug 1;327(2):C387-C402

Role of Rho/MRTF in Aggressive Vemurafenib-Resistant Murine Melanomas and Immune Checkpoint Upregulation.

Foda BM, Neubig RR
International journal of molecular sciences 2023 Sep 7;24(18)

Myc-dependent dedifferentiation of Gata6(+) epidermal cells resembles reversal of terminal differentiation.

Bernabé-Rubio M, Ali S, Bhosale PG, Goss G, Mobasseri SA, Tapia-Rojo R, Zhu T, Hiratsuka T, Battilocchi M, Tomás IM, Ganier C, Garcia-Manyes S, Watt FM
Nature cell biology 2023 Oct;25(10):1426-1438

Different Mouse Models of Nemaline Myopathy Harboring Acta1 Mutations Display Differing Abnormalities Related to Mitochondrial Biology.

Tinklenberg JA, Slick RA, Sutton J, Zhang L, Meng H, Beatka MJ, Vanden Avond M, Prom MJ, Ott E, Montanaro F, Heisner J, Toro R, Hardeman EC, Geurts AM, Stowe DF, Hill RB, Lawlor MW
The American journal of pathology 2023 Oct;193(10):1548-1567

Aberrations in Energetic Metabolism and Stress-Related Pathways Contribute to Pathophysiology in the Neb Conditional Knockout Mouse Model of Nemaline Myopathy.

Slick RA, Tinklenberg JA, Sutton J, Zhang L, Meng H, Beatka MJ, Vanden Avond M, Prom MJ, Ott E, Montanaro F, Heisner J, Toro R, Granzier H, Geurts AM, Stowe DF, Hill RB, Lawlor MW
The American journal of pathology 2023 Oct;193(10):1528-1547

The well-developed actin cytoskeleton and Cthrc1 expression by actin-binding protein drebrin in myofibroblasts promote cardiac and hepatic fibrosis.

Hironaka T, Takizawa N, Yamauchi Y, Horii Y, Nakaya M
The Journal of biological chemistry 2023 Mar;299(3):102934

Liu L, Sun K, Luo Y, Wang B, Yang Y, Chen L, Zheng S, Wu T, Xiao P
Life sciences 2023 Mar 15;317:121470

MKL1 fuels ROS-induced proliferation of vascular smooth muscle cells by modulating FOXM1 transcription.

Wu T, Li N, Zhang Q, Liu R, Zhao H, Fan Z, Zhuo L, Yang Y, Xu Y
Redox biology 2023 Feb;59:102586

An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling.

Kann AP, Hung M, Wang W, Nguyen J, Gilbert PM, Wu Z, Krauss RS
Cell stem cell 2022 Jun 2;29(6):933-947.e6

Differential regulation of cranial and cardiac neural crest by serum response factor and its cofactors.

Dinsmore CJ, Soriano P
eLife 2022 Jan 19;11

Progerin mislocalizes myocardin-related transcription factor in Hutchinson-Guilford Progeria syndrome.

von Kleeck R, Castagnino P, Assoian RK
Vascular biology (Bristol, England) 2022 Feb 1;4(1):1-10

Resident Fibroblast MKL1 Is Sufficient to Drive Pro-fibrogenic Response in Mice.

Huang S, Shao T, Liu H, Li T, Gui X, Zhao Q
Frontiers in cell and developmental biology 2021;9:812748

The Jumonji Domain-Containing Histone Demethylase Homolog 1D/lysine Demethylase 7A (JHDM1D/KDM7A) Is an Epigenetic Activator of RHOJ Transcription in Breast Cancer Cells.

Zhang Z, Chen B, Zhu Y, Zhang T, Zhang X, Yuan Y, Xu Y
Frontiers in cell and developmental biology 2021;9:664375

MRTF-A-NF-κB/p65 axis-mediated PDL1 transcription and expression contributes to immune evasion of non-small-cell lung cancer via TGF-β.

Du F, Qi X, Zhang A, Sui F, Wang X, Proud CG, Lin C, Fan X, Li J
Experimental & molecular medicine 2021 Sep;53(9):1366-1378

Hypoxia induces stress fiber formation in adipocytes in the early stage of obesity.

Anvari G, Bellas E
Scientific reports 2021 Nov 2;11(1):21473

ERa-36 instead of ERa mediates the stimulatory effects of estrogen on the expression of viral oncogenes HPV E6/E7 and the malignant phenotypes in cervical cancer cells.

Zhang X, Zhang A, Zhang X, Hu S, Bao Z, Zhang Y, Jiang X, He H, Zhang TC
Virus research 2021 Dec;306:198602

An MRTF-A-Sp1-PDE5 Axis Mediates Angiotensin-II-Induced Cardiomyocyte Hypertrophy.

Wu T, Wang H, Xin X, Yang J, Hou Y, Fang M, Lu X, Xu Y
Frontiers in cell and developmental biology 2020;8:839

MicroRNA-143-5p modulates pulmonary artery smooth muscle cells functions in hypoxic pulmonary hypertension through targeting HIF-1α.

Tang BI, Tang MM, Xu QM, Guo JL, Xuan L, Zhou J, Wang XJ, Zhang H, Kang PF
Journal of biosciences 2020;45

Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.

Dong X, Feng M, Yang H, Liu H, Guo H, Gao X, Liu Y, Liu R, Zhang N, Chen R, Kong R
International journal of biological sciences 2020;16(15):2835-2852

MRTF-A promotes angiotensin II-induced inflammatory response and aortic dissection in mice.

Ito S, Hashimoto Y, Majima R, Nakao E, Aoki H, Nishihara M, Ohno-Urabe S, Furusho A, Hirakata S, Nishida N, Hayashi M, Kuwahara K, Fukumoto Y
PloS one 2020;15(3):e0229888

Chen B, Li Z, Feng Y, Wu X, Xu Y
Biochemical and biophysical research communications 2018 Sep 10;503(3):1732-1739

The actin-MRTF-SRF transcriptional circuit controls tubulin acetylation via α-TAT1 gene expression.

Fernández-Barrera J, Bernabé-Rubio M, Casares-Arias J, Rangel L, Fernández-Martín L, Correas I, Alonso MA
The Journal of cell biology 2018 Mar 5;217(3):929-944

The Wnt-β-catenin signaling regulated MRTF-A transcription to activate migration-related genes in human breast cancer cells.

He H, Du F, He Y, Wei Z, Meng C, Xu Y, Zhou H, Wang N, Luo XG, Ma W, Zhang TC
Oncotarget 2018 Mar 16;9(20):15239-15251

Epigenetic activation of PERP transcription by MKL1 contributes to ROS-induced apoptosis in skeletal muscle cells.

Li Z, Chen B, Dong W, Xu W, Song M, Fang M, Guo J, Xu Y
Biochimica et biophysica acta. Gene regulatory mechanisms 2018 Jul 27;

The Synergy of Two Factors on Insulin Expression.

Luo Y, Xu Y, Wang ZY, Li X, Xing WB, Zhang TC
Cellular reprogramming 2018 Feb;20(1):49-54

MRTF-A mediates the activation of COL1A1 expression stimulated by multiple signaling pathways in human breast cancer cells.

Meng C, He Y, Wei Z, Lu Y, Du F, Ou G, Wang N, Luo XG, Ma W, Zhang TC, He H
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Aug;104:718-728

Transcription of HOTAIR is regulated by RhoC-MRTF-A-SRF signaling pathway in human breast cancer cells.

He H, Wei Z, Du F, Meng C, Zheng D, Lai Y, Yao H, Zhou H, Wang N, Luo XG, Ma W, Zhang TC
Cellular signalling 2017 Feb;31:87-95

Transcriptional factors p300 and MRTF-A synergistically enhance the expression of migration-related genes in MCF-7 breast cancer cells.

He H, Wang D, Yao H, Wei Z, Lai Y, Hu J, Liu X, Wang Y, Zhou H, Wang N, Luo XG, Zhang TC
Biochemical and biophysical research communications 2015 Nov 27;467(4):813-20

Histone acetyltransferase p300 promotes MRTF-A-mediates transactivation of VE-cadherin gene in human umbilical vein endothelial cells.

Shu XZ, Zhang LN, Zhang R, Zhang CJ, He HP, Zhou H, Wang N, Zhang TC
Gene 2015 May 25;563(1):17-23

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The MKL1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human MKL1. This antibody recognizes human antigen. The MKL1 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 21166-1-AP.pdf

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