PAB22486

antibody from Abnova Corporation

Targeting: MRTFA BSAC, KIAA1438, MAL, MKL, MKL1, MRTF-A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22486

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22486, RRID:AB_10966029

Product name

MKL1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant MKL1.

Antigen sequence

EQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQP
LGPAHPFNPSLAAPATNHIDPCAVAPGPPSVVVKQ
EALQPEPEPVPAPQLLLGPQGPSLIKGVAPPTLIT
DSTGTHLVLTVTNKNADSPG


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with MKL1 polyclonal antibody (Cat # PAB22486) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with MKL1 polyclonal antibody (Cat # PAB22486) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta with MKL1 polyclonal antibody (Cat # PAB22486) shows strong nuclear positivity in decidual cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)