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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- PA5-26964 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.25 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TAF2 in lysates from HT-29, Jurkat, KG-1, PC-3 cell line (from left to right). Samples were incubated with TAF2 polyclonal antibody (Product # PA5-26964) using a dilution of 1:1,000 followed by goat anti-rabbit IgG H&L (HRP) at a dilution of 1:5,000. Lysates at 35 µg per lane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TAF2 in formalin fixed and paraffin embedded human lung carcinoma. Samples were incubated with TAF2 polyclonal antibody (Product # PA5-26964) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TAF2 in formalin fixed and paraffin embedded human lung carcinoma. Samples were incubated with TAF2 polyclonal antibody (Product # PA5-26964) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MCF-7 cells using a TAF2 polyclonal antibody (Product # PA5-26964) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TAF2 in MCF-7 cells (right histogram). Samples were incubated with TAF2 polyclonal antibody (Product # PA5-26964) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
