A303-782A

antibody from Invitrogen Antibodies

Targeting: RPRD1B C20orf77, CREPT, dJ1057B20.2, DKFZp434P0735, FLJ44520, K-H, Kub5-Hera, NET60

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A303-782A

Provider

Invitrogen Antibodies

Product name

RPRD1B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RPRD1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPRD1B antibody A303-782A (lot A303-782A-2) used for IP at 3 µg per reaction. RPRD1B was also immunoprecipitated by rabbit anti-RPRD1B antibody BL13606. For blotting immunoprecipitated RPRD1B, A303-782A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse RPRD1B by western blot. Samples:Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody:Affinity purified rabbit anti-RPRD1B antibody A303-782A (lot A303-782A-2) used for WB at 0.1 µg/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.