PA5-148238

antibody from Invitrogen Antibodies

Targeting: RPRD1B C20orf77, CREPT, dJ1057B20.2, DKFZp434P0735, FLJ44520, K-H, Kub5-Hera, NET60

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-148238

Provider

Invitrogen Antibodies

Product name

RPRD1B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

100 µg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of RPRD1B in Hela cells. Samples were incubated with RPRD1B polyclonal antibody (Product # PA5-148238) using a dilution of 1:100 at 4° overnight followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, and blocked with 10% serum. Positive staining was localized to Nucleus.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of RPRD1B in various lysates. Samples were incubated with RPRD1B polyclonal antibody (Product # PA5-148238) using a dilution of 1:100 followed by Goat Anti-Rabbit IgG (H+L)/HRP secondary antibody at a dilution of 1:10,000. Lane A: 0.5 mg HeLa Whole Cell Lysate; Lane B: 0.5 mg Jurkat Whole Cell Lysate; Lane C: 0.5 mg HeLa Whole Cell Lysate; Lane D: 0.5 mg HepG2 Whole Cell Lysate. 4 µL anti- RPRD1B rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 38 kDa; Observed band size: 38 kDa.