PA5-148238
antibody from Invitrogen Antibodies
Targeting: RPRD1B
C20orf77, CREPT, dJ1057B20.2, DKFZp434P0735, FLJ44520, K-H, Kub5-Hera, NET60
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- PA5-148238 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPRD1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 100 µg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of RPRD1B in Hela cells. Samples were incubated with RPRD1B polyclonal antibody (Product # PA5-148238) using a dilution of 1:100 at 4° overnight followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, and blocked with 10% serum. Positive staining was localized to Nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of RPRD1B in various lysates. Samples were incubated with RPRD1B polyclonal antibody (Product # PA5-148238) using a dilution of 1:100 followed by Goat Anti-Rabbit IgG (H+L)/HRP secondary antibody at a dilution of 1:10,000. Lane A: 0.5 mg HeLa Whole Cell Lysate; Lane B: 0.5 mg Jurkat Whole Cell Lysate; Lane C: 0.5 mg HeLa Whole Cell Lysate; Lane D: 0.5 mg HepG2 Whole Cell Lysate. 4 µL anti- RPRD1B rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 38 kDa; Observed band size: 38 kDa.