PA5-31614
antibody from Invitrogen Antibodies
Targeting: RPRD1B
C20orf77, CREPT, dJ1057B20.2, DKFZp434P0735, FLJ44520, K-H, Kub5-Hera, NET60
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-31614 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPRD1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, HepG2. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (83%), Chicken (93%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPRD1B Polyclonal Antibody detects RPRD1B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPRD1B protein stained by RPRD1B Polyclonal Antibody (Product # PA5-31614) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPRD1B Polyclonal Antibody detects RPRD1B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPRD1B protein stained by RPRD1B Polyclonal Antibody (Product # PA5-31614) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RPRD1B was performed in paraffin-embedded mouse cervix tissue using RPRD1B Polyclonal Antibody (Product # PA5-31614) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RPRD1B (Product # PA5-31614) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using RPRD1B (Product # PA5-31614) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.