15252-1-AP

antibody from Invitrogen Antibodies

Targeting: MAGED2 11B6, BCG1, HCA10, JCL-1, MAGE-D2, MAGED, MGC8386

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15252-1-AP

Provider

Invitrogen Antibodies

Product name

MAGED2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DEPEPESAA AQSQENQDTR PKVKAKKARK VKHLDGEEDG SSDQSQASGT TGGRRVSKAL MASMARRASR GPIAFWARRA SRTRLAAWAR RALLSLRSPK ARRGKARRRA AKLQSSQEPE APPPRDVALL QGRANDLVKY LLAKDQTKIP IKRSDMLKDI IKEYTDVYPE IIERAGYSLE KVFGIQLKEI DKNDHLYILL STLEPTDAGI LGTTKDSPKL GLLMVLLSII FMNGNRSSEA VIWEVLRKLG LRPGIHHSLF GDVKKLITDE FVKQKYLDYA RVPNSNPPEY EFFWGLRSYY ETSKMKVLKF ACKVQKKDPK EWAAQYREAM (151-479 aa encoded by BC000304)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MAGED2 antibody (Product # 15252-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), F-actin was stained using CoraLite®594-phalloidin (red) and DNA was stained by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using MAGED2 antibody 15252-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MAGED2 (IP:15252-1-AP, 4ug; Detection:15252-1-AP 1:1000) with HepG2 cells lysate 3200ug.