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Western blot
Immunohistochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-47352 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HBEGF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) Amphiregulin, rhBetacellulin, rhEpiregulin, and recombinant mouse Epigen is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 250 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HBEGF in immersion fixed paraffin-embedded sections of human placenta. Samples were incubated in HBEGF polyclonal antibody (Product # PA5-47352) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HBEGF in immersion fixed paraffin-embedded sections of human placenta. Samples were incubated in HBEGF polyclonal antibody (Product # PA5-47352) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of HBEGF in Balb/3T3 mouse embryonic fibroblast cell line. Samples were incubated in HBEGF polyclonal antibody (Product # PA5-47352). Recombinant Human HB‚EGF stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB‚EGF (20 ng/mL) is neutralized (green line) by increasing concentrations of Human HB‚EGF Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.1-0.6 µg/mL.
