MA5-35756

antibody from Invitrogen Antibodies

Targeting: TMPRSS2 PRSS10

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-35756

Provider

Invitrogen Antibodies

Product name

TMPRSS2 Recombinant Rabbit Monoclonal Antibody (7B9W3)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: MALNSGSPPA IGPYYENHGY QPENPYPAQP TVVPTVYEVH PAQYYPSPVP QYAPRVLTQA SNPVVCTQPK SPSGTVCTSK TKKALCITLT LGTFLVGAAL

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

7B9W3

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Transmembrane serine protease 2 is a prognostic factor for lung adenocarcinoma.

Schneider MA, Richtmann S, Gründing AR, Wrenger S, Welte T, Meister M, Kriegsmann M, Winter H, Muley T, Janciauskiene S
International journal of oncology 2022 Apr;60(4)

---

miR-98 Regulates TMPRSS2 Expression in Human Endothelial Cells: Key Implications for COVID-19.

Matarese A, Gambardella J, Sardu C, Santulli G
Biomedicines 2020 Oct 30;8(11)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CRISPR-Cas9 mediated genome editing ofTMPRSS2 (as confirmed by next generation sequencing) was achieved by using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR883619_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Fig (a) Western blot analysis of TMPRSS2 was performed by loading 50 µg of LNCaP wild type (Lane 1), LNCaP Cas9 (Lane 2) and LNCaP Cas9 cells transduced with TMPRSS2 Lentiviral sgRNA (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with TMPRSS2 Recombinant Rabbit Monoclonal Antibody (ARC1439) (Product # MA5-35756, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution).Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). A reduced signal in sgRNA transduced cells using the LentiArray™ CRISPR product line confirms that antibody is specific toTMPRSS2 (Fig (b)).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TMPRSS2 performed on various cell lines using a TMPRSS2 monoclonal antibody (Product # MA5-35756) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression and correlation of TMPRSS2 and AAT in NSCLC patients. (A) Western blot analyses of TMPRSS2 and AAT in 12 SQCC and ADC patient samples (N, normal and T, tumor tissues). Tissues were homogenized and 100 u g of each sample was used for immunoblot analysis. (B) Quantification of TMPRSS2 and AAT protein expression comparing expression values of tumor (T) and normal (N) tissues in SQCC and ADC (fold change in %). (C and D) Correlation of TMPRSS2 and AAT protein expression in normal and tumor tissues of patient samples [(C) SQCC; (D) ADC] from A. Values in the frames are shown in more detail using correlation plots. r>0.5 was considered as an adequate correlation. TMPRSS2, transmembrane serine protease 2; AAT, alpha-1-antitrypsin; NSCLC, non-small cell lung cancer; ADC, adenocarcinoma; SQCC, squamous cell carcinoma.