A300-974A

antibody from Invitrogen Antibodies

Targeting: ANKRD28 CFAP79, FAP79, KIAA0379, PITK, PP6-ARS-A, PPP1R65

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-974A

Provider

Invitrogen Antibodies

Product name

ANKRD28/PITK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ANKRD28/PITK by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa cells (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ANKRD28/PITK antibody A300-974A used for WB at 0.02 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). ANKRD28/PITK was also immunoprecipitated by rabbit anti-ANKRD28/PITK antibody A300-975A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ANKRD28/PITK by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ANKRD28/PITK (Cat. No. A300-974A Lot1) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB.