Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- AF3885 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Lipin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Lipin 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) Lipin 2 and rhLipin 3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q14693
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references HIF-1-dependent lipin1 induction prevents excessive lipid accumulation in choline-deficient diet-induced fatty liver.
Degradation of human Lipin-1 by BTRC E3 ubiquitin ligase.
Dietary cholesterol reduces plasma triacylglycerol in apolipoprotein E-null mice: suppression of lipin-1 and -2 in the glycerol-3-phosphate pathway.
Sterol-mediated regulation of human lipin 1 gene expression in hepatoblastoma cells.
Arai T, Tanaka M, Goda N
Scientific reports 2018 Sep 21;8(1):14230
Scientific reports 2018 Sep 21;8(1):14230
Degradation of human Lipin-1 by BTRC E3 ubiquitin ligase.
Ishimoto K, Hayase A, Kumagai F, Kawai M, Okuno H, Hino N, Okada Y, Kawamura T, Tanaka T, Hamakubo T, Sakai J, Kodama T, Tachibana K, Doi T
Biochemical and biophysical research communications 2017 Jun 17;488(1):159-164
Biochemical and biophysical research communications 2017 Jun 17;488(1):159-164
Dietary cholesterol reduces plasma triacylglycerol in apolipoprotein E-null mice: suppression of lipin-1 and -2 in the glycerol-3-phosphate pathway.
Obama T, Nagaoka S, Akagi K, Kato R, Horiuchi N, Horai Y, Aiuchi T, Arata S, Yamaguchi T, Watanabe M, Itabe H
PloS one 2011;6(8):e22917
PloS one 2011;6(8):e22917
Sterol-mediated regulation of human lipin 1 gene expression in hepatoblastoma cells.
Ishimoto K, Nakamura H, Tachibana K, Yamasaki D, Ota A, Hirano K, Tanaka T, Hamakubo T, Sakai J, Kodama T, Doi T
The Journal of biological chemistry 2009 Aug 14;284(33):22195-205
The Journal of biological chemistry 2009 Aug 14;284(33):22195-205
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Lipin 1 in Human Adipocytes. Lipin 1 was detected in immersion fixed human mesenchymal stem cell-derived adipocytes using Goat Anti-Human Lipin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3885) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.