MA5-24206

antibody from Invitrogen Antibodies

Targeting: STRA6 FLJ12541

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24206

Provider

Invitrogen Antibodies

Product name

STRA6 Monoclonal Antibody (496613)

Antibody type

Monoclonal

Antigen

Other

Description

Reconstitute at 0.5 mg/mL in sterile PBS.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

496613

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of STRA6 in immersion fixed Raji human Burkitts lymphoma cell line. Samples were incubated in STRA6 monoclonal antibody (Product # MA5-24206) using a dilution of 3 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of STRA6 in immersion fixed Raji human Burkitts lymphoma cell line. Samples were incubated in STRA6 monoclonal antibody (Product # MA5-24206) using a dilution of 3 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HT-29 human colon adenocarcinoma cell line was stained with human STRA6 Monoclonal Antibody (Product # MA5-24206) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-mouse IgG F (ab')2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of STRA6 in HT‚29 human colon adenocarcinoma cell line. Samples were incubated in STRA6 monoclonal antibody (Product # MA5-24206) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.