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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- R33305 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Sprouty Antibody / SPRY1
- Antibody type
- Polyclonal
- Description
- This highly specific Sprouty antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Immunocytochemistry/Flow cytometry/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the Sprouty antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human kidney lysate with Sprouty antibody at 0.3ug/ml. Predicted molecular weight ~35 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC testing of fixed and permeabilized human HeLa cells with Sprouty antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC testing of fixed and permeabilized human HepG2 cells with Sprouty antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human HEK293 cells with Sprouty antibody (blue) at 10ug/10^6 cells and naive goat Ig (black).
