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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB6097 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SPRY1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human SPRY1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human SPRY2, 3, or 4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O43609- Isotype
- IgG
- Antibody clone number
- 669534
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human SPRY1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and LNCaP human prostate cancer cell line. PVDF Membrane was probed with 0.5 µg/mL of Human SPRY1 Monoclonal Antibody (Catalog # MAB6097) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SPRY1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
