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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [3]
- Immunoprecipitation [3]
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- Product number
- LS-C197613 - Provider product page
- Provider
- LSBio
- Product name
- LGALS1 / Galectin 1 Antibody (clone 1E8-1B2) LS-C197613
- Antibody type
- Monoclonal
- Description
- Purified from ascites by Protein A
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1E8-1B2
- Storage
- Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- LGALS1 monoclonal antibody (M01), clone 1E8-1B2 Western Blot analysis of LGALS1 expression in HepG2.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot analysis of LGALS1 expression in transfected 293T cell line by LGALS1 monoclonal antibody (M01), clone 1E8-1B2.Lane 1: LGALS1 transfected lysate(14.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection limit for recombinant GST tagged LGALS1 is 1 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged LGALS1 is 1 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged LGALS1 is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of LGALS1 transfected lysate using anti-LGALS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LGALS1 rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of LGALS1 transfected lysate using anti-LGALS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LGALS1 rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of LGALS1 transfected lysate using anti-LGALS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LGALS1 rabbit polyclonal antibody.
