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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Western blot [5]
- Immunocytochemistry [3]
- Immunoprecipitation [1]
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- Product number
- GTX116411 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116411, RRID:AB_10624275
- Product name
- Galectin 1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Treatment of B-cell precursor acute lymphoblastic leukemia with the Galectin-1 inhibitor PTX008.
Binding of Galectin-3, a β-Galactoside-binding Lectin, to MUC1 Protein Enhances Phosphorylation of Extracellular Signal-regulated Kinase 1/2 (ERK1/2) and Akt, Promoting Tumor Cell Malignancy.
Biomarker discovery for neuroendocrine cervical cancer.
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Paz H, Joo EJ, Chou CH, Fei F, Mayo KH, Abdel-Azim H, Ghazarian H, Groffen J, Heisterkamp N
Journal of experimental & clinical cancer research : CR 2018 Mar 27;37(1):67
Journal of experimental & clinical cancer research : CR 2018 Mar 27;37(1):67
Binding of Galectin-3, a β-Galactoside-binding Lectin, to MUC1 Protein Enhances Phosphorylation of Extracellular Signal-regulated Kinase 1/2 (ERK1/2) and Akt, Promoting Tumor Cell Malignancy.
Mori Y, Akita K, Yashiro M, Sawada T, Hirakawa K, Murata T, Nakada H
The Journal of biological chemistry 2015 Oct 23;290(43):26125-40
The Journal of biological chemistry 2015 Oct 23;290(43):26125-40
Biomarker discovery for neuroendocrine cervical cancer.
Lin LH, Chang SJ, Hu RY, Lin MW, Lin ST, Huang SH, Lyu PC, Chou HC, Lai ZY, Chuang YJ, Chan HL
Electrophoresis 2014 Jul;35(14):2039-45
Electrophoresis 2014 Jul;35(14):2039-45
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
May EW, Lin ST, Lin CC, Chang JF, Hung E, Lo YW, Lin LH, Hu RY, Feng CL, Lin DY, Wu SB, Lee WC, Lyu KW, Chou HC, Chan HL
Pharmacological research 2014 Dec;90:1-17
Pharmacological research 2014 Dec;90:1-17
Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Fan CY, Chou HC, Lo YW, Wen YF, Tsai YC, Huang H, Chan HL
Electrophoresis 2013 Dec;34(24):3305-14
Electrophoresis 2013 Dec;34(24):3305-14
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NCI-H929 15% SDS PAGE GTX116411 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: BCL-1 15% SDS PAGE GTX116411 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Galectin 1 antibody detects Galectin 1 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract B. 30 ?g Rat2 whole cell lysate/extract15% SDS-PAGEGalectin 1 antibody (GTX116411) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Galectin 1 knockout (KO) 293T cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using Galectin 1(GTX116411) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Galectin 1 antibody detects Galectin 1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Galectin 1 antibody immunoprecipitates Galectin 1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 £gg whole cell lysate/extract of Galectin 1 antibody expressing HeLa cells B : Control with 2.5 £gg of pre-immune rabbit IgG C : Immunoprecipitation of Galectin 1 by 2.5 £gg of Galectin 1 antibody (GTX116411) 15% SDS-PAGE The immunoprecipitated Galectin 1 protein was detected by Galectin 1 antibody (GTX116411) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
