Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [6]
- Immunohistochemistry [9]
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Validation data
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- Product number
- LS-C344090 - Provider product page
- Provider
- LSBio
- Product name
- LGALS1 / Galectin 1 Antibody (aa2-135) LS-C344090
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody Western blot. All lanes: Anti Galectin1 at 0.5 ug/ml. WB: Recombinant Human Galectin1 Protein 0.5ng. Predicted band size: 16 kD. Observed band size: 16 kD.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody Western blot. All lanes: Anti Galectin1 at 0.5 ug/ml. Lane 1: Rat Testis Tissue Lysate at 50 ug. Lane 2: Rat Brain Tissue Lysate at 50 ug. Lane 3: Rat Liver Tissue Lysate at 50 ug. Lane 4: Rat Kidney Tissue Lysate at 50 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Lane 6: SMMC Whole Cell Lysate at 40 ug. Lane 7: NRK Whole Cell Lysate at 40 ug. Predicted band size: 15 kD. Observed band size: 15 kD.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody ICC: NRK Cell.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- anti-Galectin1 antibodyICCICC: A549 Cell
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ICC analysis of Galectin 1 using anti-Galectin 1 antibody. Galectin 1 was detected in immunocytochemical section of rat NRK cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Galectin 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Galectin1 antibody ICC: NRK Cell.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- anti-Galectin1 antibodyICCICC: A549 Cell
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ICC analysis of Galectin 1 using anti-Galectin 1 antibody. Galectin 1 was detected in immunocytochemical section of rat NRK cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Galectin 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody IHC-paraffin: Rat Cardiac Muscle Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody IHC-paraffin: Mouse Cardiac Muscle Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody IHC-paraffin: Human Placenta Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody IHC-frozen: Human Placenta Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody IHC-paraffin: Rat Cardiac Muscle Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody IHC-frozen: Human Placenta Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody IHC-paraffin: Mouse Cardiac Muscle Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody IHC-paraffin: Human Placenta Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Galectin1 antibody IHC-frozen: Human Placenta Tissue.