Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-7618 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-Galectin-1 Antibody
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Full length recombinant Galectin-1
protein was used as the immunogen f
or this antibody.- Isotype
- IgG
- Antibody clone number
- ABM5B54
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of Galectin-1. Anti-Galectin-1 antibody (Clone: ABM5B54) was tested at 0.01 µg/ml on (1) Recombinant protein and 2 µg/ml on (2) Hela, (3) 3T3, (4) PC3, (5) 293, (6) THP1, (7) K562 and (8) HCT-116 lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Galectin-1. Anti-Galectin-1 (Clone: ABM5B54) was used in Colon Carcinoma tissue at 2 µg/ml.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- 3- Intracellular flow analysis of Galectin-1 in A431 cell line using 0.5 µg/10^6 cells of Galectin-1 antibody (Clone: ABM5B54). Green represents isotype control; red represents anti-Galectin-1 antibody (10-7618). Goat anti-mouse PE conjugate was used as secondary antibody.
- Protocol
- Protocol