PA5-19665

antibody from Invitrogen Antibodies

Targeting: LGALS1 GBP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19665

Provider

Invitrogen Antibodies

Product name

Anti-Galectin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse, rat, sheep, cow, pig and orangutan based on sequence homology. Store antibody at 4ºC for 1-2 weeks. For long-term storage, store at -20ºC.

Reactivity

Human, Hamster

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.7 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Human Heart Tissue Lysate using Product # PA5-19665, Galectin 1 primary antibody at a dilution of 1 µg/mL. Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of Galectin 1 (LGALS1) was achieved by transfecting PC-3 with LGALS1 specific siRNAs (Silencer® select Product # s194592, s8145). Western blot analysis (Fig. a) was performed using whole cell extracts from the Galectin 1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Galectin 1 Polyclonal Antibody (Product # PA5-19665, 0.7 µg/mL dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Galectin 1.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Galectin 1 Polyclonal Antibody (Product # PA5-19665) and a 15 kDa band corresponding to Galectin 1 was observed across cell lines tested except in MCF7 and LNCaP which are reported low expressing for the same. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1), LNCaP (Lane 2), THP-1 (Lane 3), MCF7 (Lane 4) and MDA-MB-231 (Lane 5) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.7 µg/mL dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).