PA5-30706

antibody from Invitrogen Antibodies

Targeting: LGALS1 GBP

Western blot

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-30706 - Provider product page
Provider: Invitrogen Antibodies
Product name: Galectin 1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Recommended positive controls: K562, THP-1, HL-60, 293T, NCI-H929, BCL-1, PC-12, Rat2. Predicted reactivity: Mouse (88%), Rat (90%), Dog (82%), Pig (85%), Sheep (85%), Rhesus Monkey (97%), Bovine (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.48 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

LGALS1 protein structure - PA5-30706 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Galectin 1 in methanol-fixed A431 cells using a Galectin 1 polyclonal antibody (Product # PA5-30706) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Galectin 1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin 1 Polyclonal Antibody (Product # PA5 30706) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Galectin 1 was performed in A549 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin 1 Polyclonal Antibody (Product # PA5 30706) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Galectin 1 was performed using 70% confluent log phase MDA-MB-231 and MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Galectin 1 Polyclonal Antibody (Product # PA5-30706) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization of Galectin 1 in MDA-MB-231. Panel e represents MCF7 cells showing negative staining for the same. Panel f represents control MDA-MB-231 cells with no primary antibody to assess background. The images were captured at 60X magnification.