Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BAF1152 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Galectin-1 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Galectin-1 in Western blots. In this format, approximately 20% cross-reactivity with recombinant mouse Galectin-1 is observed and less than 5% cross-reactivity with recombinant human (rh) Galectin-2, rhGalectin-4, rhGalectin-7, and rhGalectin-8 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P09382- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Serum galectin-1 in patients with multiple myeloma: associations with survival, angiogenesis, and biomarkers of macrophage activation.
Mesenchymal stem cell-mediated T cell suppression occurs through secreted galectins.
Andersen MN, Ludvigsen M, Abildgaard N, Petruskevicius I, Hjortebjerg R, Bjerre M, Honoré B, Møller HJ, Andersen NF
OncoTargets and therapy 2017;10:1977-1982
OncoTargets and therapy 2017;10:1977-1982
Mesenchymal stem cell-mediated T cell suppression occurs through secreted galectins.
Sioud M, Mobergslien A, Boudabous A, Fløisand Y
International journal of oncology 2011 Feb;38(2):385-90
International journal of oncology 2011 Feb;38(2):385-90
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Galectin-1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1152) followed by Streptavidin-HRP. A specific band was detected for Galectin-1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
