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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- 15475-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE2D4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MALKRIQKE LTDLQRDPPA QCSAGPVGDD LFHWQATIMG PNDSPYQGGV FFLTIHFPTD YPFKPPKVAF TTKIYHPNIN SNGSICLDIL RSQWSPALTV SKVLLSICSL LCDPNPDDPL VPEIAHTYKA DREKYNRLAR EWTQKYAM (1-147 aa encoded by BC004104)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 15475-1-AP (UBE2D4 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 15475-1-AP (UBE2D4 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 15475-1-AP) (UBE2D1/2/3/4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
