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LearnHPA028885
antibody from Atlas Antibodies
Targeting: PHF5A
bK223H9.2, INI, MGC1346, Rds3, SAP14b, SF3b14b, SF3B7
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- HPA028885 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA028885, RRID:AB_10602079
- Product name
- Anti-PHF5A
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human PHF5A, Gene description: PHD finger protein 5A, Alternative Gene Names: bK223H9.2, INI, MGC1346, Rds3, SAP14b, SF3b14b, SF3B7, Validated applications: WB, IHC, ICC, Uniprot ID: Q7RTV0, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references PHF5A promotes colorectal cancer progression by alternative splicing of TEAD2
An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses
Chang Y, Zhao Y, Wang L, Wu M, He C, Huang M, Lei Z, Yang J, Han S, Wang B, Chen Y, Liu C, Yu H, Xue L, Geng J, Chen Y, Dai T, Ren L, Wang Q, Liu X, Chu X, Chen C
Molecular Therapy - Nucleic Acids 2021;26
Molecular Therapy - Nucleic Acids 2021;26
An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses
Hearing P, Schreiber C, Sakuma T, Izumiya Y, Holditch S, Hickey R, Bressin R, Basu U, Koide K, Asokan A, Ikeda Y
PLOS Pathogens 2015;11(8):e1005082
PLOS Pathogens 2015;11(8):e1005082
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Enhanced validation
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting PHF5A or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting PHF5A Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:58
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PHF5A antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
