PA5-56004

antibody from Invitrogen Antibodies

Targeting: PHF5A bK223H9.2, INI, MGC1346, Rds3, SAP14b, SF3b14b, SF3B7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56004

Provider

Invitrogen Antibodies

Product name

PHF5A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AKHHPDLIFC RKQAGVAIGR LCEKCDGKCV ICDSYVRPCT LVRICDECNY GSYQGRCVIC GGPGVSDAYY CKECTIQEKD RDGCPKIVNL GSSKTDLFYE RK

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PHF5A in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a PHF5A Polyclonal Antibody (Product # PA5-56004). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PHF5A in control (vector only transfected HEK293T lysate) and PHF5A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a PHF5A Polyclonal Antibody (Product # PA5-56004).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PHF5A in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a PHF5A Polyclonal Antibody (Product # PA5-56004).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PHF5A in human duodenum tissue shows nuclear positivity in glandular cells. Samples were probed using a PHF5A Polyclonal Antibody (Product # PA5-56004).