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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-29373 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLK4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PLK4-transfected 293T.
- Concentration
- 0.19 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLK4 using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PLK4 polyclonal antibody (Product # PA5-29373) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PLK4 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PLK4 Polyclonal Antibody (Product # PA5-29373) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PLK4 in methanol-fixed HeLa cells using a PLK4 polyclonal antibody (Product # PA5-29373) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PLK4 Polyclonal Antibody detects PLK4 protein at cytoplasm and nucleus in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. PLK4 Polyclonal Antibody (Product # PA5-29373) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PLK4 Polyclonal Antibody detects PLK4 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. PLK4 Polyclonal Antibody (Product # PA5-29373) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
