Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-894 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-894, RRID:AB_2284148
- Product name
- Goat Polyclonal PLK4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Polo-like kinase 1 is involved in invasion through extracellular matrix.
Sak kinase gene structure and transcriptional regulation.
Sak kinase gene structure and transcriptional regulation.
Rizki A, Mott JD, Bissell MJ
Cancer research 2007 Dec 1;67(23):11106-10
Cancer research 2007 Dec 1;67(23):11106-10
Sak kinase gene structure and transcriptional regulation.
Hudson JW, Chen L, Fode C, Binkert C, Dennis JW
Gene 2000 Jan 4;241(1):65-73
Gene 2000 Jan 4;241(1):65-73
Sak kinase gene structure and transcriptional regulation.
Hudson JW, Chen L, Fode C, Binkert C, Dennis JW
Gene 2000 Jan 4;241(1):65-73
Gene 2000 Jan 4;241(1):65-73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PLK4 Antibody [NB100-894] - Analysis of PLK4 in human Colon lysate (35ug protein in RIPA buffer) using NB100-894 at 2 ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PLK4 Antibody [NB100-894] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.