Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00010509-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010509-M03, RRID:AB_581764
- Product name
- SEMA4B monoclonal antibody (M03), clone 4B2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant SEMA4B.
- Antigen sequence
GEKPCEQVQFQPNTVNTLACPLLSNLATRLWLRNG
APVNASASCHVLPTGDLLLVGTQQLGEFQCWSLEE
GFQQLVASYCPEVVEDGVADQTDEGGSV- Isotype
- IgG
- Antibody clone number
- 4B2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SEMA4B expression in transfected 293T cell line by SEMA4B monoclonal antibody (M03), clone 4B2.Lane 1: SEMA4B transfected lysate (Predicted MW: 10.89 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SEMA4B is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
