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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
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- Product number
- PA5-31796 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEMA4B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, mouse brain. Predicted reactivity: Mouse (86%), Rat (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SEMA4B Polyclonal Antibody (Product # PA5-31796). Sample (30 µg of whole cell lysate). Lane A: U87-MG . 7.5% SDS PAGE. SEMA4B Polyclonal Antibody (Product # PA5-31796) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SEMA4B Polyclonal Antibody (Product # PA5-31796). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. SEMA4B Polyclonal Antibody (Product # PA5-31796) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U373 xenograft, using SEMA4B (Product # PA5-31796) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEMA4B Polyclonal Antibody detects SEMA4B protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. SEMA4B Polyclonal Antibody (Product # PA5-31796) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEMA4B Polyclonal Antibody detects SEMA4B protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. SEMA4B Polyclonal Antibody (Product # PA5-31796) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEMA4B Polyclonal Antibody detects SEMA4B protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. SEMA4B Polyclonal Antibody (Product # PA5-31796) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
