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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- MAB5485 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Semaphorin 4B Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Semaphorin 4B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Semaphorin 4B is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9NPR2- Isotype
- IgG
- Antibody clone number
- 561416
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Semaphorin 4B in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human Semaphorin 4B Monoclonal Antibody (Catalog # MAB5485, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
