Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-5867-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Integrin beta 7 Monoclonal Antibody (FIB504), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The FIB504 monoclonal antibody reacts with human and mouse integrin beta 7. Integrin beta 7 is a 130 kD glycoprotein which associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Main ligands for integrin alpha 4 beta 7 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the main ligand of integrin alpha E beta 7 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response. The FIB504 antibody has been reported to have blocking activity. Applications Reported: This FIB504 antibody has been reported for use in flow cytometric analysis and functional assays. Applications Tested: This FIB504 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- FIB504
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Protection from septic peritonitis by rapid neutrophil recruitment through omental high endothelial venules.
Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases.
Differential gene expression by integrin beta 7+ and beta 7- memory T helper cells.
Engagement of alpha4beta7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro.
Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation.
Buscher K, Wang H, Zhang X, Striewski P, Wirth B, Saggu G, Lütke-Enking S, Mayadas TN, Ley K, Sorokin L, Song J
Nature communications 2016 Mar 4;7:10828
Nature communications 2016 Mar 4;7:10828
Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases.
Hovhannisyan Z, Treatman J, Littman DR, Mayer L
Gastroenterology 2011 Mar;140(3):957-65
Gastroenterology 2011 Mar;140(3):957-65
Differential gene expression by integrin beta 7+ and beta 7- memory T helper cells.
Rodriguez MW, Paquet AC, Yang YH, Erle DJ
BMC immunology 2004 Jul 5;5:13
BMC immunology 2004 Jul 5;5:13
Engagement of alpha4beta7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro.
Meerschaert J, Vrtis RF, Shikama Y, Sedgwick JB, Busse WW, Mosher DF
Journal of immunology (Baltimore, Md. : 1950) 1999 Dec 1;163(11):6217-27
Journal of immunology (Baltimore, Md. : 1950) 1999 Dec 1;163(11):6217-27
Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation.
Andrew DP, Berlin C, Honda S, Yoshino T, Hamann A, Holzmann B, Kilshaw PJ, Butcher EC
Journal of immunology (Baltimore, Md. : 1950) 1994 Nov 1;153(9):3847-61
Journal of immunology (Baltimore, Md. : 1950) 1994 Nov 1;153(9):3847-61
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.06 µg of Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.06 µg of Anti-Human/Mouse Integrin β7 Purified (filled histogram) followed by F (ab')2 Anti-Rat IgG PE (Product # 12-4822).Cells in the lymphocyte gate were used for analysis.