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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- NB600-244 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-244, RRID:AB_10001248
- Product name
- Rabbit Polyclonal ZHX1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Early changes in gene expression that influence the course of primary glomerular disease.
ZHX proteins regulate podocyte gene expression during the development of nephrotic syndrome.
Clement LC, Liu G, Perez-Torres I, Kanwar YS, Avila-Casado C, Chugh SS
Kidney international 2007 Aug;72(3):337-47
Kidney international 2007 Aug;72(3):337-47
ZHX proteins regulate podocyte gene expression during the development of nephrotic syndrome.
Liu G, Clement LC, Kanwar YS, Avila-Casado C, Chugh SS
The Journal of biological chemistry 2006 Dec 22;281(51):39681-92
The Journal of biological chemistry 2006 Dec 22;281(51):39681-92
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZHX1 Antibody [NB600-244] - Nuclear extract (NE) from HeLa cells. Antibody used at 0.2 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZHX1 Antibody [NB600-244] - Detection of Human ZHX1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZHX1 antibody NB600-244 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZHX1 Antibody [NB600-244] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZHX1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ZHX1 Antibody [NB600-244] - Detection of human ZHX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZHX1 antibody NB600-244 used for IP at 3 ug per reaction. ZHX1 was also immunoprecipitated by another rabbit anti-ZHX1 antibody. For blotting immunoprecipitated ZHX1, NB600-244 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZHX1 Antibody [NB600-244] - FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-ZHX1 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ZHX1 Antibody [NB600-244] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- ZHX1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZHX1 Antibody [NB600-244] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- ZHX1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
