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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [1]
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- Product number
- 14-0429-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD42b Monoclonal Antibody (HIP1), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb. Applications Reported: The HIP1 antibody has been reported for use in flow cytometric analysis, in vitro blocking of human CD42b, immunoblotting (WB), and immunohistochemical staining. (Please use Functional Grade purified HIP1, cat. 16-0429, in functional assays.). Applications Tested: The HIP1 antibody has been tested by flow cytometric analysis of human platelets and can be used less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibodies be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HIP1
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Platelet-derived HMGB1 is a critical mediator of thrombosis.
Identification of inherited macrothrombocytopenias based on mean platelet volume among patients diagnosed with idiopathic thrombocytopenia.
Staphylococcus aureus adhesion via Spa, ClfA, and SdrCDE to immobilized platelets demonstrates shear-dependent behavior.
Influence of monoclonal antiplatelet glycoprotein antibodies on in vitro human megakaryocyte colony formation and proplatelet formation.
Vogel S, Bodenstein R, Chen Q, Feil S, Feil R, Rheinlaender J, Schäffer TE, Bohn E, Frick JS, Borst O, Münzer P, Walker B, Markel J, Csanyi G, Pagano PJ, Loughran P, Jessup ME, Watkins SC, Bullock GC, Sperry JL, Zuckerbraun BS, Billiar TR, Lotze MT, Gawaz M, Neal MD
The Journal of clinical investigation 2015 Dec;125(12):4638-54
The Journal of clinical investigation 2015 Dec;125(12):4638-54
Identification of inherited macrothrombocytopenias based on mean platelet volume among patients diagnosed with idiopathic thrombocytopenia.
Gohda F, Uchiumi H, Handa H, Matsushima T, Tsukamoto N, Morita K, Amagai H, Murakami M, Murakami H, Nojima Y, Karasawa M
Thrombosis research 2007;119(6):741-6
Thrombosis research 2007;119(6):741-6
Staphylococcus aureus adhesion via Spa, ClfA, and SdrCDE to immobilized platelets demonstrates shear-dependent behavior.
George NP, Wei Q, Shin PK, Konstantopoulos K, Ross JM
Arteriosclerosis, thrombosis, and vascular biology 2006 Oct;26(10):2394-400
Arteriosclerosis, thrombosis, and vascular biology 2006 Oct;26(10):2394-400
Influence of monoclonal antiplatelet glycoprotein antibodies on in vitro human megakaryocyte colony formation and proplatelet formation.
Takahashi R, Sekine N, Nakatake T
Blood 1999 Mar 15;93(6):1951-8
Blood 1999 Mar 15;93(6):1951-8
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of human platelets with Anti-Human CD42b FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total cells were used for analysis.
