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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- H00009700-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009700-M01, RRID:AB_538649
- Product name
- Mouse Monoclonal Separase Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ESPL1 - extra spindle poles like 1 (S. cerevisiae)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Separase is a marker for prognosis and mitotic activity in breast cancer.
MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ERα)-positive mammary adenocarcinomas.
Identification and Characterization of Separase Inhibitors (Sepins) for Cancer Therapy.
Overexpression and constitutive nuclear localization of cohesin protease Separase protein correlates with high incidence of relapse and reduced overall survival in glioblastoma multiforme.
Separase loss of function cooperates with the loss of p53 in the initiation and progression of T- and B-cell lymphoma, leukemia and aneuploidy in mice.
Calpain-1 cleaves Rad21 to promote sister chromatid separation.
Overexpression and mislocalization of the chromosomal segregation protein separase in multiple human cancers.
Overexpression of Separase induces aneuploidy and mammary tumorigenesis.
Gurvits N, Löyttyniemi E, Nykänen M, Kuopio T, Kronqvist P, Talvinen K
British journal of cancer 2017 Oct 24;117(9):1383-1391
British journal of cancer 2017 Oct 24;117(9):1383-1391
MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ERα)-positive mammary adenocarcinomas.
Mukherjee M, Ge G, Zhang N, Edwards DG, Sumazin P, Sharan SK, Rao PH, Medina D, Pati D
Oncogene 2014 Nov 27;33(48):5511-5522
Oncogene 2014 Nov 27;33(48):5511-5522
Identification and Characterization of Separase Inhibitors (Sepins) for Cancer Therapy.
Zhang N, Scorsone K, Ge G, Kaffes CC, Dobrolecki LE, Mukherjee M, Lewis MT, Berg S, Stephan CC, Pati D
Journal of biomolecular screening 2014 Jul;19(6):878-89
Journal of biomolecular screening 2014 Jul;19(6):878-89
Overexpression and constitutive nuclear localization of cohesin protease Separase protein correlates with high incidence of relapse and reduced overall survival in glioblastoma multiforme.
Mukherjee M, Byrd T, Brawley VS, Bielamowicz K, Li XN, Merchant F, Maitra S, Sumazin P, Fuller G, Kew Y, Sun D, Powell SZ, Ahmed N, Zhang N, Pati D
Journal of neuro-oncology 2014 Aug;119(1):27-35
Journal of neuro-oncology 2014 Aug;119(1):27-35
Separase loss of function cooperates with the loss of p53 in the initiation and progression of T- and B-cell lymphoma, leukemia and aneuploidy in mice.
Mukherjee M, Ge G, Zhang N, Huang E, Nakamura LV, Minor M, Fofanov V, Rao PH, Herron A, Pati D
PloS one 2011;6(7):e22167
PloS one 2011;6(7):e22167
Calpain-1 cleaves Rad21 to promote sister chromatid separation.
Panigrahi AK, Zhang N, Mao Q, Pati D
Molecular and cellular biology 2011 Nov;31(21):4335-47
Molecular and cellular biology 2011 Nov;31(21):4335-47
Overexpression and mislocalization of the chromosomal segregation protein separase in multiple human cancers.
Meyer R, Fofanov V, Panigrahi A, Merchant F, Zhang N, Pati D
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Apr 15;15(8):2703-10
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Apr 15;15(8):2703-10
Overexpression of Separase induces aneuploidy and mammary tumorigenesis.
Zhang N, Ge G, Meyer R, Sethi S, Basu D, Pradhan S, Zhao YJ, Li XN, Cai WW, El-Naggar AK, Baladandayuthapani V, Kittrell FS, Rao PH, Medina D, Pati D
Proceedings of the National Academy of Sciences of the United States of America 2008 Sep 2;105(35):13033-8
Proceedings of the National Academy of Sciences of the United States of America 2008 Sep 2;105(35):13033-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Separase Antibody (6H6) [H00009700-M01] - ESPL1 monoclonal antibody (M01), clone 6H6 Analysis of ESPL1 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Separase Antibody (6H6) [H00009700-M01] - Analysis of monoclonal antibody to ESPL1 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.
