MA1-16595

antibody from Invitrogen Antibodies

Targeting: ESPL1 ESP1, KIAA0165, SEPA

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-16595

Provider

Invitrogen Antibodies

Product name

Separase Monoclonal Antibody (XJ11-1B12)

Antibody type

Monoclonal

Antigen

Other

Description

This Separase (XJ11-1B12) antibody is useful for Western Blot, where a band is seen at ~220 kDa (full-length form) and ~65 kDa (auto-processed form).

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

XJ11-1B12

Vial size

100 µL

Concentration

0.9 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

The proteolytic activity of separase in BCR-ABL-positive cells is increased by imatinib.

Haaß W, Stehle M, Nittka S, Giehl M, Schrotz-King P, Fabarius A, Hofmann WK, Seifarth W
PloS one 2012;7(8):e42863

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Transcript levels, protein levels and proteolytic activity of Separase in BCR-ABL-positive cells treated with IM. Cells were treated individually with distinct concentrations (0.5 to 5 uM) of IM. After about two cell cycle rounds (K562, LAMA-84, 24 h; U937p210BCR-ABL/c6-On, 48 h) total RNA and protein lysates were prepared and analyzed by qRT-PCR (A), Western blot immunostaining (B) and separase fluorometric activity assays (C). For Western blot experiments, Actin served as loading control and/or for densitometric data normalization. Each data point corresponds to one single experiment. Only significant p-values as calculated between treated and untreated cells were shown (see Table 2 for summarized Delta-values). For a representative set of corresponding immunostained Western blots compare Figure 5 panel C. Abbreviations: U937/c6-On, U937 cells expressing a p210BCR-ABL transgene (Tet-On system) after induction with Doxycycline (U937p210BCR-ABL/c6-On).