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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-12691 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Separase (Ser1126) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.25 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-Separase (Ser1126) in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with Phospho-Separase (Ser1126) polyclonal antibody (Product # PA5-12691) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Dot blot analysis of Phospho-Separase (Ser1126). Samples were incubated with Phospho-Separase (Ser1126) polyclonal antibody (Product # PA5-12691) using a dilution of 0.5 µg/mL. 50 ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots on nitrocellulose membrane.
