TA503440

antibody from OriGene

Targeting: PGAM2 PGAM-M

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503440

Provider

OriGene

Proper citation

OriGene Cat#TA503440, RRID:AB_11142689

Product name

PGAM2 mouse monoclonal antibody, clone OTI4B12 (formerly 4B12)

Antibody type

Monoclonal

Description

PGAM2 mouse monoclonal antibody, clone OTI4B12 (formerly 4B12)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

PGAM2

Isotype

IgG

Antibody clone number

OTI4B12

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-PGAM2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PGAM2 monoclonal antibody. (1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PGAM2 mouse monoclonal antibody (TA503440) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2(RC200701).

Validation comment

IF