MA5-25672

antibody from Invitrogen Antibodies

Targeting: PGAM2 PGAM-M

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-25672

Provider

Invitrogen Antibodies

Product name

PGAM2 Monoclonal Antibody (OTI4B12)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

OTI4B12

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PGAM2 in 3T3 cells, mouse brain, mouse liver, mouse spleen lysate using 10 µg per lane. Samples were probed with PGAM2 (Product # MA5-25672) monoclonal antibody at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PGAM2 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with PGAM2 (Product # MA5-25672) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PGAM2 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with PGAM2 (Product # MA5-25672) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PGAM2 in human tissue (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon) samples using 10 µg per lane. Samples were separated by SDS-PAGE and probed with PGAM2 (Product # MA5-25672) monoclonal antibody at a dilution of 1:200.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PGAM2 in COS7 cells. Cells were transfected with a plasmid overexpressing PGAM2 and probed with a PGAM2 monoclonal antibody (Product # MA5-25672).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PGAM2 in COS7 cells. Cells were transfected with a plasmid overexpressing PGAM2 and probed with a PGAM2 monoclonal antibody (Product # MA5-25672).