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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-22038 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGAM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: H1299, HeLa, rat muscle. Predicted reactivity: Mouse (92%), Rat (93%), Zebrafish (86%), Xenopus laevis (86%), Pig (96%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PGAM2 Polyclonal Antibody (Product # PA5-22038). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: Hela. 12% SDS PAGE. PGAM2 Polyclonal Antibody (Product # PA5-22038) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PGAM2 Polyclonal Antibody detects PGAM2 protein by Western blot analysis. A. 50 µg rat muscle lysate/extract.12 % SDS-PAGE. PGAM2 Polyclonal Antibody (Product # PA5-22038) dilution: 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PGAM2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PGAM2 Polyclonal Antibody (Product # PA5-22038) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
