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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- MAB5260 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-28 R alpha/IFN-lambda R1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-28 R alpha/IFN-lambda R1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-28 alpha is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8IU57- Isotype
- IgG
- Antibody clone number
- 601106
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-28 R alpha/IFN-lambda R1 in PBMCs by Flow Cytometry. Peripheral blood mononuclear cells (PBMCs) either unstimulated (A) or treated (B) with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and Mouse Anti-Human IL-28 R alpha/IFN-lambda R1 Monoclonal Antibody (Catalog # MAB5260) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-28 R alpha/IFN-lambda R1 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-28 R alpha/IFN-lambda R1 Monoclonal Antibody (Catalog # MAB5260, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
