PA5-91959

antibody from Invitrogen Antibodies

Targeting: FBXO32 ATROGIN1, Fbx32, MAFbx

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-91959

Provider

Invitrogen Antibodies

Product name

FBXO32 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: WQQQLNNIQI TRPAFKGLTF TDLPLCLQLN IMQRLSDGRD LVSLGQAAPD LHVLSEDRLL WKKLCQYHFS ERQIRKRLIL SDKGQLDWKK MYFKLVRCYP RKEQYGDTLQ LCKHCHILSW KGTDHPCTAN NPESCSVSLS PQDFINLFKF

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.35 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Effects of Root Extract of Morinda officinalis in Mice with High-Fat-Diet/Streptozotocin-Induced Diabetes and C2C12 Myoblast Differentiation.

Wang P, Liu Y, Zhang T, Yin C, Kang SY, Kim SJ, Park YK, Jung HW
ACS omega 2021 Oct 19;6(41):26959-26968

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Improvement of muscular atrophy by AAV-SaCas9-mediated myostatin gene editing in aged mice.

Weng S, Gao F, Wang J, Li X, Chu B, Wang J, Yang G
Cancer gene therapy 2020 Dec;27(12):960-975

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of FBXO32 in extracts of various cell lines using FBXO32 Polyclonal Antibody (Product # PA5-91959) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of FBXO32 in mouse skeletal muscle. Samples were incubated with FBXO32 Polyclonal antibody (Product # PA5-91959) using a dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Effect of MORE on the muscle tissue in the mice with HFD/STZ-induced diabetes. (A) Expressions of MHC, MyoD, myogenin, PGC-1alpha, and Atrogin1 were determined by Western blot. (B-F) Relative expression of each protein was normalized to beta-actin. The expression of each protein was measured by Western blotting and compared with the expression of beta-actin as an internal control. The data are expressed as the mean +- S.D. ( n = 5). Nor, normal group; Control, groups with HFD/STZ-induced diabetes; MORE100, group administered 100 mg/kg MORE; MORE200, group administered 200 mg/kg MORE; and Met, group administered 250 mg/kg metformin. # p < 0.05, ## p < 0.01, and ### p < 0.001 vs Nor; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs Control.