ATC-010-200UL

antibody from Invitrogen Antibodies

Targeting: TMC1 DFNA36, DFNB11, DFNB7

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ATC-010-200UL

Provider

Invitrogen Antibodies

Product name

TMC1 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis ofhuman THP-1monocyticleukemia cell line lysate (lanes 1 and4),human HL-60promyelocyticleukemia cell line lysate (lanes 2 and5)andhuman 132N1 astrocytoma cell line lysate (lanes 3 and 6): - 1-3. Anti-TMC1 (extracellular) Antibody (#ATC-010), (1:200).4-6. Anti-TMC1 (extracellular) Antibody ,preincubated with TMC1 (extracellular)Blocking Peptide (BLP-TC010).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse colon lysate (lanes 1 and3)and rat eye lysate (lanes 2 and4): - 1-2. Anti-TMC1 (extracellular) Antibody (#ATC-010), (1:200).3-4. Anti-TMC1 (extracellular) Antibody ,preincubated with TMC1 (extracellular)Blocking Peptide (BLP-TC010).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection ofTMC1by indirect flow cytometry in live intacthuman THP-1monocyticleukemiacell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-TMC1 (extracellular) Antibody (#ATC-010), (2.5μg) + goat- Anti-rabbit-FITC.