Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB7848 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Collybistin/ARHGEF9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Collybistin/ARHGEF9 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O43307
- Isotype
- IgG
- Antibody clone number
- 809309
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Presynaptic Neuronal Pentraxin Receptor Organizes Excitatory and Inhibitory Synapses.
Lee SJ, Wei M, Zhang C, Maxeiner S, Pak C, Calado Botelho S, Trotter J, Sterky FH, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Feb 1;37(5):1062-1080
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Feb 1;37(5):1062-1080
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Collybistin/ARHGEF9 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, human brain (cortex) tissue, and mouse brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Collybistin/ARHGEF9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Collybistin/ARHGEF9 in Human Brain. Collybistin/ARHGEF9 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.