LS-B10380

antibody from LSBio

Targeting: VSNL1 HLP3, HPCAL3, HUVISL1, VILIP, VILIP-1

Western blot (Enhanced validation)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B10380

Provider

LSBio

Product name

IHC-plus™ VILIP / VSNL1 Antibody (clone 2D11) LS-B10380

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat, Bovine

Host

Mouse

Isotype

IgG

Antibody clone number

2D11

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of bovine cerebellum homogenate stained with VILIP / VSNL1 antibody in lane 10. Note the strong clean band running at 22kDa. Lane 11 shows the same material stained with an alternate antibody to VSNL1, which binds to the same band.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using antibody at a dilution of 1:1000. Lane 1, marker; Lane 2, rat brain; Lane 3, mouse brain; Lane 4, pig hippocampus; and Lane 5, bovine cerebellum.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Confocal image of adult rat cerebellar cortex stained with VILIP / VSNL1 antibody (green), chicken polyclonal antibody to MAP2 (red) and DNA (blue). The VILIP / VSNL1 antibody antibody reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) are revealed with MAP2 antibody (4). Little staining is seen in the white matter (WM).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Confocal image of adult rat cerebellar cortex stained with VILIP / VSNL1 antibody (green), chicken polyclonal antibody to MAP2 (red) and DNA (blue). The VILIP / VSNL1 antibody antibody reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) are revealed with MAP2 antibody (4). Little staining is seen in the white matter (WM).