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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- NBP2-25155 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal LAMP-1/CD107a Antibody
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LAMP-1/CD107a Antibody (6E2) [NBP2-25155] - Western strip blots of HeLa cell crude extracts with two different preparations of NBP2-25155 in lanes 17 and 18. Both preparations bind to a diffuse band running at between ~90kDa and ~120kDa as expected, and show no appreciably cross reactivity with any other protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (6E2) [NBP2-25155] - HeLa cells stained with NBP2-25155 (green), and counterstained with a chicken polyclonal antibody to Lamin A + C (red) and DNA (blue). NBP2-25155 reveals strong cytoplasmic staining of lysosomes and early endosomes, while the Lamin A + C antibody binds to the nuclear lamina. Since both DNA (blue) and Lamin A + C (red) are associated with the nuclear compartment, this region appears crimson in this image.
