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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB100-1952-100ug - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1952-100ug, RRID:AB_11017632
- Product name
- Mouse Monoclonal LAMP-1/CD107a Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 120kDa.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: LAMP-1/CD107a Antibody (LY1C6) [NB100-1952] - analysis of Rat liver microsome lysate showing detection of LAMP1 protein using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
